Molecular Devices ThermoMax is a high-efficiency, temperature-regulated, 96-well microplate reader. albicans pre. Filipin StainingSpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro data analysis software. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Title: SPECTRAmax 340 Microplate Reader manual Author: Research Facilities Keywords: spectramax, 340, microplate reader, manual, Created Date: 12/13/2005 9:07:53 AMSpectraMax Multi-Mode Microplate Readers. Urinary proteins were measured using a Micro BCA assay kit following the manufacturer’s instructions (Thermo Fisher Scientific, Rockford, IL, USA). 05% at 340 nm Photometric stabilization Instantaneous Photometric drift None—continuous referencing of monochromatic output Calibration Automatic before first kinetic read and. Lightgreenglass,2 mmthick(340 nm) 9:Yellowglass,3 mmthick(405 nm) 10:Darkredglass,3 mmthick(650 nm)automated SpectraMAX 340 microtiter plate reader. The optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. Products : SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax 250 (Obsolete), SpectraMax 340 (Obsolete), VersaMax reader How does stray light affect my OD reading? The basic problem caused by stray light (light you don't want, getting into the read) is a falsely low OD seen. 7. All strains were tested three times and the results averaged. using SpectraMax i3 (Molecular devices, San Jose, USA). The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Then, the absorbance value at 450 nm was determined using a microplate reader (SpectraMAX 340; Molecular Devices, Sunnyvale, CA, United States). The microtiter plate was then incubated in the dark for 2 h at 37°C. SPECTRAmax® 190. Lactate Dehydrogenase (LDH) Release. Each experiment was performed in duplicate and repeated at. D. 3. SpectraMax ® Plus 384 optics. The SpectraMax i3x reader provides the flexibility to add novel detection capabilities without the need for service engineers or costly downtime—it grows with you as your research areas expand. , Sunnyvale, CA). Molecular Devices Spectramax 340PC instructions. spectramax i3 multi-mode platform, spectramax i3, spectramax i3 system, scanlater western blot system, spectramax minimax imaging cytometer, softmax pro software, metamorph software, spectramax i3 platform. The reduction of HED in the presence of NADPH and GR was recorded at 340 nm. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. SpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro data analysis software. One plate carrier adapter is provided with the instrument. IC 50 values were determined by mathematical curve-fitting (SoftMaxPro Molecular Devices) and were defined as the drug concentration leading to 50% inhibition of net cell mass. Continuous kinetic monitoring of enzyme activity was performed on SPECTRAmax 340 or Flexstation 2-384 microplate reader (Molecular Devices, Sunnyvale, CA, USA) and controlled by the Softmax software. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. Inter-assay variation was prevented by testing all of the samples on the same day. addresses the needs of life science researchers who quantify very small amounts of DNA, RNA, oligos, and proteins. FXII or PK activation by FXIa was tested as follows: FXII or PK (200nM) was incubated with or without FXIa (25nM) in TBS, 0. Description 1. The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Novak, Nathan J. Like CD3, the CD95 death receptor requires binding of immobilized or multimeric antibodies to be activated effectively. After adding the stop solution, the absorbance at 450 nm with a wavelength correction at 540 nm was measured with a SpectraMax 340 plate reader (Molecular Devices, San Jose, CA, USA). Quick View. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. CD154 detection in equine PBMC by an anti-human CD154 antibody (clone 5C8) was confirmed by Western blots and then applied for flow cytometry. The concentration of Alizarin Red S staining in the samples was determined by comparing the absorbance values with those. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. With SOFTmax PRO, the contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, making it possible to. 2. The sensitivities of the hormones are Prolactin: 0. Briefly, cells were seeded into a. 5. 2 M DEEMM were mixed in a microtube,. Absorbance was measured at 490/650 nm by using a SpectraMax 340 microplate spectrophotometer® (Molecular Devices, Sunnyvale, CA, U. MOLECULAR DEVICE M5. Availablility: By Availability Molecular Devices SpectraMAX 340 Microplate Reader. According to LDH assay, a 60 μL of reaction mixture containing catalyst (diaphorase/NAD+), dye solution of iodotetrazolium chloride and sodium lactate was. Subsequently, the mixture was heated at 100 °C for 10. Wound healing assay. #N207) LAL Reagent Water (Lonza cat. One plate carrier adapter is provided with the instrument. CSF-1, IL-34 and calprotectin levels were analysed by ELISA according to instructions provided by the manufacturer (R&D Systems, Minneapolis, Minnesota, USA). Donor filter: 620 nm. 313 reference gain check fail. , Oxon, UK). Total Cell Count in BALF Cells collected from the lavage fluid were suspended in 1 mL of F-12 media supplemented with 10% FBS. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. After washing, the dye was eluted with 3% HCl and the absorbance measured at 665 nm with a SpectraMax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA). Products : SpectraMax Paradigm reader, SpectraMax Plus 384 reader, SpectraMax 250 (Obsolete), SpectraMax L reader, SpectraMax 340PC 384 (Obsolete), Gemini EM reader, Gemini XPS reader, SpectraMax 340 (Obsolete). Thin layer chromatography (TLC) was. 5. The absorbance at 540 nm was determined in a spectrophotometer (SPECTRAmax 340, Molecular Devices). These robust readers have been placed in labs from Antarctica to the. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Acceptor: 665/10 nm. Apoptosis was also measured by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) assay using the TACS Blue label kit (4811-30-K, R&D Systems). The values were compared against a standard curve that was generated using known concentrations of IL6 to calculate concentration in the. Molecular Devices SpectraMax 384 Plus Microplate Reader Specifications. Homeostatic model assessment for insulin. In this study we used tetanus toxoid (TT) as a well-defined antigen to. 05% at 340 nm Photometric stabilization Instantaneous Photometric drift None—continuous referencing of monochromatic output Calibration Automatic before first kinetic read and. 4- to 5-weeks old female BALB/c mice were purchased from Charles River Laboratories (QC, Canada). Molecular Device SpectraMax M3 Plate Reader - Certified with Warranty. The absorbance was measured at 340 nm for 2 min at a 15 s time interval. Photometric Performance SpectraMax 340PC384 Item Description Wavelength range 340 nm to 850 nm Wavelength selection Monochromator tunable in 1 nm increments Wavelength bandwidth. After 24 h, 48 h, 72 h or 96 h, cell viability was measured by CellTiter 96® AQueous One Solution cell proliferation assay (Promega, Madison, WI, USA). When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. We selected the response at t=10 h for statistical analysis, because at that time point the response variation was largest. 1x10 5 transduced, GFP + SK-NEP-1. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. If depot service is the best option for you we will assist you with logistics to help you keep. The adapter is. The mixture changed to a red color by a reaction of LDH that was measured by a plate reader (SpectraMax 340 PC; Molecular Devices, San Jose, CA, USA) at a visible wavelength of. ABS reader ranges from 340-850 nm in wavelength and the ABS Plus reader ranges from 190-1000 nm, which is equivalent to having 510 or 810 free filters, respectively. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Details. Or scan up to 384 wells at any time to determine the best assay conditions. Introduction The SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature. Stat Fax 4700 Microstrip Reader. All assays were done in triplicate with readings at 60-s intervals using a SpectraMax 340 multiwell plate reader (Molecular Devices, San Jose, CA, United States). Pharmacokinetic and pharmacodynamic analysis. 30. The first time you replace an OEM lamp you must use the N-34-MLA. 2 Chemical Reagents 1. Details. The cell death was calculated as follows:Absorbance was monitored at 450 nm (SpectraMax 340). They are for reference only. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytes. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. This light offers spectrum variability, wireless control, and photosensors to automatically balance output with natural light. The SpectraMax ABS Plus, can accommodate standard 96-well plates and 384-well plates. 4) was followed according to Vanacker et al. Bioz Stars score: 86/100, based on 1 PubMed citations. The absorbances were being measured at 515 nm. 5 mL of 1 M acetate buffer (pH 4. a SpectraMAX 340VR microplate reader (Molecular Devices, Sunnyvale, CA, USA). 00 USD. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. Nitric Oxide Assay. After adding the stop solution, the absorbance at 450 nm with a wavelength correction at 540 nm was measured with a SpectraMax 340 plate reader (Molecular Devices, San. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. Hydrogen peroxide (H 2 O 2) was used to establish an intracellular oxidative. Page: 1/6. 305 reference level saturation. SpectraMax® Microplate Readers and SoftMax® Pro Software. Number of flashes. Each experiment was performed in duplicate and repeated at. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. , San Jose, CA) using Softmax Pro 7 software. 0. Features demonstrated by the model include temperature control, a robot-compatible interface and data analysis software. , Fremont, CA, USA). The plate was covered and incubated for one hour at room temperature. The optical density of each sample was analyzed at 450 nm with a reference reading at 630 nm using a SpectraMax 340 absorbance plate reader (Molecular Devices, Union City, CA). Figure 1. For use with Spectramax 340 devices which have an 8-pin mini DIN serial socket. Below is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. After then, 10% of ammonium hydroxide solution was added to neutralize the aqueous solution of the ARS extracts, and the absorbance values were measured using a SpectraMax® 340 plate reader at 405 nm. Absorbance was read at 405 nm on a microplate reader (SPECTRAmax 340 PC). 316 grating motor driver faulty. 00. Integration delay. Related Products: SpectraMax 340 Absorbance Microplate Reader, Molecular Devices SpectraMax 384 Plus UV/Vis Microplate Reader, Molecular DevicesGETTING THE MOST FROM YOUR MOLECULAR DEVICES MICROPLATE SYSTEMS applications guide to microplate systems applications guide to microplate systems450 nm using a SpectraMax 340 microplate reader (Molecular Devices, Sunnyvale, CA, USA). Customer Transformation Hub. SpectraMax Plus 384 measuring instruments pdf manual download. Isolation of RNA and RT-PCR. 304 signal level saturation. The SpectraMax® iD3 Multi Mode Microplate . For FXII, at various times, 90 μL of reaction was mixed with SBTI (500nM) and S-2302 (0. Molecular Devices SpectraMax M5e Microplate Reader. At that point halfway up the maximum transmittance value, the total width (nm) of the transmitted band is. The optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. Photometric Range: 0. Intra- and inter-assay coefficients of variation were lower than 8. 340/80 nm excitation with 616/10 nm emission range Optimized for use with ScanLater Western Blot Detection cartridge for the SpectraMax® i3, i3x, and Paradigm® Multi-Mode Readers Optimized for use with the enhanced TRF detection module for the SpectraMax iD5 Multi-Mode Microplate ReaderThe optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. After the addition of sPLA2 (20 μ g), the reaction mixture was incubated for 40 min at 37°C, and the absorbance was read at 10 min intervals. The SPECTRAmax 340PC can mea- TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytes acrossawiderangeofconcentrations. Molecular Devices Spectramax 340PC instructions. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. SOD activities were calculated from assays in which there was an inhibition of cytochrome c reduction of between 40 and 50%. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Absorbance measurements were recorded using a SpectraMax 340 (Molecular Devices) at 490 nm on the indicated days (Cell Proliferation Kit I; Roche). On-site Repair Service available nationwide for Molecular Devices Spectramax 340 Plate Reader's. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). The plates were read using an ELISA microplate reader (Molecular Devices SPECTRAMAX 340 PC 384, Sunnyvale, California, USA) at 450 nm. The results of ELISA optical density (OD) values in duplicates were averaged for the following analyses. SpectraMax® M2/M2e user guide a multi-detection microplate reader with two-mode cuvette port SpectraMax M2e user guide cover 1 1 4/21/06 9:53:28 AM. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Absorbance values were read at 570 nm on an automated SpectraMAX 340 (Molecular Devices, Sunnyvale, CA, USA). Scanning was done at 600 nm, using a Molecular Devices SpectraMax 340 plate reader. SpectraMax 340PC384 reads 96-well plates and 384-well plates and strip-well plates. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Microplate Reader Shelf. The slope of the linear portion of the kinetic curve generated from each well was used to. Details. Furthermore, the stain was extracted using 10% acetic acid, and its absorbance was measured at 595 nm in a microtiter plate reader (SpectraMax 340; Molecular Devices). Samples with OD values of less than 0. This Molecular Devices SPECTRAmax 340PC incorporates a holographic grating. Optical density was determined at 490 nm (background values measured at 690 nm were subtracted) using a SpectraMax 340 microplate reader (Molecular Devices, Sunnyvale, CA, USA). The results are expressed as the percentage inhibition of BrdU incorporation compared to the untreated control. ). Molecular Devices SpectraMax Gemini XS Microplate Reader, 250-850nm, Molecular Devices, for detection of samples in microplates. These lab workhorses are used in drug discovery, research, bioassay validation, and. The optical density of each well was determined immediately using a microplate reader (SpectraMax-340, Molecular Devices) set to 450 nm. Not all manufacturers' microplates are the same with regard to design, materials, or76358-624. SpectraMAX 340. The release of p-nitrophenol ( 400) 17000 M-1 cm-1) was monitored at 25 °C for the enzymatic hydrolysis of com-pounds 1-8. Call 1-800-745-2710. The absorbance values were measured at 450 nm using a SpectraMax® 340 plate reader (Molecular Devices, Sunnyvale, CA, USA). NO release assayThe SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. Created Date: 20051213090753Z measured using a SpectraMax 340 multiwell plate. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. Features showcased by this model include temperature control, a robotics-compatible interface and Data Analysis Software. 2. When you do a read at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeOpening the Drawer Manually on page The SpectraMax ABS can accommodate standard 96-well plates. Oxidative stress induction by H 2 O. 5), L-ascorbic acid (25. Irene Tieleman, Ido PenandSimon VerhulstSubsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. 4. SpectraMax 340 Absorbance Microplate Reader, Molecular Devices (CAT#: STEM-SPO-0021-ZJF) Labsystems Fluoroskan II Fluorescent Microplate Reader, Excitation Wavelength Range 320 – 700 nm, Labsystems (CAT#: STEM-SPO-0006-LGZ) BioTek MQX200 Microplate Reader Spectrophotometer, 24VDC, BioTek Instruments, Inc (CAT#: STEM. The LDH level in the lavage fluid was calculated based on the standard curve, nor-malized with protein concentration and expressed as ng/mg protein. using SpectraMax i3 (Molecular devices, San Jose, USA). Upgradeable modules are also available including western blot, cell imaging, and fast. MMP‑1 inhibition assay. 5), L-ascorbic acid (25. Time-resolved fluorescence was measured on the SpectraMax i3x and SpectraMax iD5 readers using preconfigured protocols in SoftMax® Pro Software (see Tables 2 and 3 for instrument-specific settings). Absorbance plate reader, temperature control, robotics-compatible, 384 well plates, 340-850 nm. The values were corrected for nonspecific background by subtracting the OD 650. SpectraMax Microplate Readers. Calculating % of total mediator release in RBL assay. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. Description 1. 0. control. 340 nm / 80 nm. or microplate reader (e. #W50-640) SpectraMax i3 or i3x Multi-Mode Microplate Reader (Molecular Devices; other SpectraMax microplate readers with fluorescence detection are also suitable) SoftMax Pro Software (Molecular Devices). Accurate quantitation The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurement in the visible range. ac. Confocal laser scanning microscopy (CLSM) Biofilms were formed on glass-bottomed Petri dishes (CELLview™; Greiner Bio-One GmbH, Frickenhausen, Germany) in the presence or absence (control biofilms) of the BIC 50 of antifungals as described above. 10× trypsin 0. Although it has been proven to be effective in various neurologic disorders, the mechanism of the treatment of traumatic brain injury (TBI) is not fully understood. Sandwich ELISAs were used to measure insulin (DY8056, R&D Systems, Minneapolis, MN, USA). In order to detect the Fc-tagged fusion proteins, goat anti-human IgG (Fc Fragment specific; Jackson ImmunoResearch Laboratories Inc, West Grove, PA, USA; Code Number 109-006-098;. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. ), which was proportional to the number of damaged cells. Enzyme activities at high concentration were analyzed using a SpectraMAX 340 plate reader (Molecular Devices) at 380 nm in the kinetic mode at 37°C. Recalcification (clotting) time was determined by monitoring absorbance at 650 nm at 10-sec intervals in a SpectraMax 340 microtiter plate reader, with onset time (the time to a linear increase in the OD, which reflects the maximal rate of formation of insoluble fibrin) set at an OD of 0. SpectraMax Multi-Mode Microplate Readers. The absorbance was measured at 405 nm on a SpectraMax 340 (Molecular Devices). Measurements of OD at 540 nm were then performed in a microtiter plate reader (SpectraMax 340 microplate reader; Molecular Devices Corp. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideMolecular Devices SpectraMax Gemini XS Microplate Fluorometer $2,800. 5% phosphoric acid) in microplates at room temperature for 10 min. The optical density (OD) at the wavelength of 450 nm was measured with a spectrometer (SpectraMax 340, Sunnyvale, CA, USA). Wavelength Selection. Since the formation of NADPH is stoichiometrically related to the oxidation of glucose-6-phosphate, measurements for glucose + fructose can be determined by the absorbance of NADPH at 340 nm. Following incubation for 30 min at room temperature, the reaction was quenched by addition of 100 μl stop solution (2 N H 2 SO 4) and absorbance was read using microplate reader (SpectraMax 340; Molecular Devices Co. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating. The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. SpectraMax 340 (Serial number starting with M) SpectraMax 340PC (Serial number starting with L) SpectraMax Plus (Serial number starting with P) Tmax Also refer. Other technologies such as imaging. When you do reads at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. Molecular Devices SpectraMax Gemini XS Microplate Reader. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. The absorbance was read at the wavelength of 570 nm using a microplate spectrophotometer (SpectraMax 340, Molecular Devices, CA, USA). When necessary, samples were. 2. Labsystems Luminoskan RT Microplate Reader. SpectraMax Multi-Mode Microplate Readers. The assay was carried out in a 96-well plate in a final volume of 100 µl containing 50 mmol/l Tris-HCl, pH 7. 1. Nitrocefin hydrolysis was monitored at 482 nm on a SpectraMax 340 UV-visible plate reader for high-throughput assays and an HP8453 UV-visible spectrophotometer for the low-throughput assays. Acceptor filter: 665/10 nm. The relative LDH release was determined as the percentage ratio of the optical densities in the media (containing CNMs at each. PathCheck SensorThe optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. The extinction coefficient at 280 nm used for determining the concentration of Rsp3690 was calculated to be 28,795 M –1 cm –1. The cell concentration was measured by a spectrometer (SpectraMax 340, Molecular Devices, USA) with an optical density at 600 nm (OD 600). On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. Number of flashes. Molecular Devices SpectraMax M5e Microplate Reader $26,500. Triton X-100 (see Note 2). Gerritsma,B. 5 mL of 1 M acetate buffer (pH 4. 2 were considered negative for IgM antibody. SpectraMax Gemini EM & FLEXstation (bottom read) 1. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. The SpectraMax ABS Plus reader was heated to 37ºC prior to setting up the. 5. Alves, Amelia K. 306 plate air cal. Up for your consideration is a Molecular Devices SpectraMax 340 Microplate Spectrophotometer Model# 340 Condition is for parts or repair. 6) and 40 μL acetylacetone, then the mixture was heated at 100 °C for 10 min to. The inhibition of ALDH activity by compounds and the IC50 curves were determined by measuring the formation of NAD(P)H spectrophotometrically at 340 nm (molar extinction coefficient of 6200 M −1 cm −1) on a Beckman DU-640 as well as a Spectramax 340 PC spectrophotometer (GMI, Ramsey, NJ, USA) using a purified. LBA Formats for Pharmacokinetic AssaysAfter treatment, 10 μl of CCK-8 was added to each well, and the incubation was continued at 37°C for 2 h in the dark. Molecular Devices SpectraMax M5e Microplate Reader. SpectraMax 340PC384 reads 96-well plates and 384-well plates and strip-well plates. 1% PEG 8000 at 37°C. No power cord or accessories included. FlexStation® 3 Multi-Mode Microplate Reader. Quick View. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 5. After 24 h, 48 h, 72 h or 96 h, cell viability was measured by CellTiter 96® AQueous One Solution cell proliferation assay (Promega, Madison, WI, USA). 0% and ± 0. 5. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. 0 3. Molecular Devices Corporation 1311. Molecular Devices. Spectramax 340pc User Manual, Hudson River Saranac Pellet Stove Manual, Alesis Midiverb 1 Manual, User Manual For Samsung Galaxy Ace Duos S6802, Di Style Manual, R150f Transmission Repair Manual, Eos5d Mark 2 Manual. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The activity of generated thrombin was measured by the amidolysis of its substrate S-2238 and theThe purple formazan crystals formed were dissolved by adding 100 μL of DMSO to each well and absorbance was read at 570 nm in a spectrophotometer [SpectraMax 340]. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. The ratio of red to green fluorescence intensity was calculated. 5 mL sample or standard solution was well mixed with 0. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. ELISA plates were read with a Spectramax 340 from Molecular Devices (Sunnyvale, CA, USA). 1% Chaps, 10 m M dithiothreitol, and 10% sucrose. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for. Permeability of the monolayer was verified by adding 1% Evans Blue dye (Thermo Fisher Scientific, Inc. ATI Note: #1: The N-34-MLA is a replacement assembly which replaces an OEM lamp. The absorbance at 450 nm was determined using a microplate reader (spectraMAX 340). The SpectraMax® 190 Absorbance Microplate Reader is the ideal equipment for many life science applications including DNA analysis. The possi-ble effect of zinc or copper on the binding of heme by A was tested as described above by using a 50 and 100 M final concentration of each metal. 6) and 0. The cell proliferation on different Ti discs was assessed by measuring the absorbance at 450 nm using an ELISA reader (SpectraMax® 340, Molecular Devices, Sunnyvale, CA). See More Microplate Reader / Microtiter Plate Reader. 05 ng/mL; progesterone: 2. Although all samples initially were analyzed at the same volume, the volume of sample. The optical system of the SpectraMax 340PC Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536. Centrifuge – Eppendorf 5417C with rotor FA 45–30–11. Subsequent replacements require only the lamp (N-34-LB) be replaced. Stat Fax 3200 Microplate Reader. SpectraMax Multi-Mode Microplate Readers. Or scan up to 384 wells at any time to determine the best assay conditions. 304 signal level saturation. The inhibition of ALDH activity by compounds and the IC50 curves were determined by measuring the formation of NAD(P)H spectrophotometrically at 340 nm (molar extinction coefficient of 6200 M −1 cm −1) on a Beckman DU-640 as well as a Spectramax 340 PC spectrophotometer (GMI, Ramsey, NJ, USA) using a purified. The reaction mixture consists of 100 mM potassium phosphate buffer (pH 7. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. The centrifuged solution was transferred to a different microtitre plate and the optical density (OD) of the change in color was measured using a plate reader (SpectraMAX 340 Tunable Microplate Reader; Molecular Devices Ltd. #N207) LAL Reagent Water (Lonza cat. SpectraMax i3x reader measures absorbance, fluorescence, & luminescence with user-upgradeable application including western blot imaging, AlphaScreen, fast kinetics along with injectors plus additional detection modes. Although it has been proven to be effective in various neurologic disorders, the mechanism of the treatment of traumatic brain injury (TBI) is not fully understood. 30. VersaMax reads 96-well and strip-well plates. With a wide range of on-board features and capabilities, thermoax is ideally suited for use in modern laboratories involved in many applications. The extreme flexibility and high sensitivity of the SpectraMax GEMINI EM make it appropriate for applications within the fields of bio-A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Statistical AnalysisConsult Molecular Devices's entire SpectraMax i3x catalogue on MedicalExpo. The SpectraMax GEMINI EM cannot be operated without the computer and SoftMax Pro software. . 6, 2 mmol/l DTT, 10 mmol/l MnCl 2 and 10 mmol/l pNPP; the PP2Cα enzyme concentration was 400nmol/l. D2. mums. IMAP ® Technology from Molecular Devices enables rapid, homogeneous, and non-radioactive assay of kinases, phosphatases, and phosphodiesterases and is suited for both assay development and high-throughput screening. As discussed below, dilution of the culture was required because cell density is not proportional to A 600 when the values are above ;0. 2. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytesacross. chen@case. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Narrow bandwidths deliver increased measurement accuracy and linearity over a wide range of assays. P. The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. For more sample throughput, the system can The optical absorption was measured at 450 nm for BUN and 490 nm for creatinine with a spectrometer (SpectraMax 340). (1988) and recorded at 412 nm. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. 5, 2 mM pNPP, and recombinant enzymes, PTP1B activities were continuously monitored on a SpectraMax 340 Microplate Reader (SpectraMax, Silicon Valley, CA, USA) at 405 nm for 2 min at 30°C. Italic and boldface type are used for emphasis. Microplate Readers. Awareness Technology, Inc. The optical density of each well was determined immediately using a microplate reader (SpectraMax-340, Molecular Devices) set to 450 nm. Stat Fax 4700 Microstrip Reader. Samples containing 25 μg of protein were separated on 12. Amino acid analysis was performed on a Pico-Tag amino acid analyzer (Wat er System) as. Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. 003 OD Stray light ≤ 0. FTIR Spectrometer: Shimadzu IRSpirit (2018)The data were acquired with a Molecular Devices SpectraMax 340 Microplate Reader (Sunnyvale, CA, USA). RESEARCH ARTICLE Effects of early-life conditions on innate immune function in adult zebra finches Merijn M. Excitation 1 340 nm Emission 1 520 nm Cut off for emission 1 495 nm Excitation 2 340 nm Emission 2 620 nm Cut off for emission 2 570 nm Sensitivity Readings 50 to 100 PMT Optimal Column Wavelenght Column Priority . 190–850 nm <± 1. The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance.